Skip to main content
. Author manuscript; available in PMC: 2018 Jul 15.
Published in final edited form as: Nat Struct Mol Biol. 2018 Jan 15;25(2):139–146. doi: 10.1038/s41594-017-0016-2

Figure 5. The CorEx motif of Sec3 is crucial for exocyst complex assembly and vesicle tethering.

Figure 5

a. Schematic diagram of Sec3 full-length and truncation constructs for ectopic targeting to mitochondria through their fusion with Tom20-mCherry. The dark block indicates the CorEx motif. b. Co-localization of all of the Tom20-mCherry-tagged Sec3 proteins with Cit1-GFP, a marker protein of yeast mitochondria. Scale bar, 2 µm. c. Co-localization of Sec5-GFP and the Tom20-mCherry-tagged Sec3 proteins. Scale bar, 2 µm. d. Co-localization of Sec4, a marker of post-Golgi secretory vesicles, and the Tom20-mCherry-tagged Sec3 proteins. Scale bar, 2 µm. At least 5 images were taken for each sample, with all cells bearing both green and red fluorescence showing similar co-localization patterns as displayed here.