A) Vitamin D, B) 25D, and C) 24,25D levels from serum of +D male and female mice, before and after Abx treatment. Values are the mean ± SEM of a total of n= 8 males or n=5 females, and data from 3 independent experiments. 2-way ANOVA with Bonferroni post-hoc test was used to test significance. *P<0.05, **P<0.01, ***P<0.001