A) Vitamin D, B) 25D and C) 24,25D levels from serum of WT and VDR KO mice. Values are the mean ± SEM of n=11–14 mice per group, 15 males and 10 females, and 2 pooled independent experiments. D) 24,25D levels from serum of WT and Rag KO. Values are the mean ± SEM of n=5 mice per group, 4 males and 6 females. E) 24,25D levels from serum of WT/WT and WT/VDR KO. Values are the mean ± SEM of n=4 recipient male mice per group. 2-way ANOVA with Bonferroni post-hoc test was used to test significance. *P<0.05, **P<0.01, ***P<0.001