A) 25D and 24,25D levels from Cyp27B1 KO and WT mice. Values are the mean ± SEM of n=12–14 mice per group, 16 males and 10 females combined from 3 independent experiments. B) 25D and 24,25D levels from +D WT and MyD88 KO mice. Values are the mean ± SEM of n=5–6 mice per group, 5 males and 6 females. 2-way ANOVA with Bonferroni post-hoc test was used to test significance. *P<0.05, **P<0.01, ***P<0.001