Skip to main content
. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Free Radic Biol Med. 2018 Apr 23;121:9–19. doi: 10.1016/j.freeradbiomed.2018.04.570

Figure 3. Detection of oxidative DNA damage in mtDNA of ara-C exposed DRG neurons.

Figure 3

Representative images show immunofluorescence of 8-oxoguanine (8-oxoG) in DRG neurons: (A) In DRG cultures exposed to ara-C for 24 h cytoplasmic punctate 8-oxoG immunoreactivity is observed (green); DRG neurons are identified by immunoreactivity of NF200 (red). 8-oxoG IF is observed also in small DRG neurons, which do not react with anti NF200 antibody (center panel, green; scale bar = 10 µm). (B) Bar graph shows percent of 8-oxoG positive DRG neurons as a function of exposure time. Values represent mean+SEM for four biological experiments. (C) Representative images of DRG neurons double stained with antibodies reacting with 8-oxoG (green) and mtDNA-binding mitochondrial transcription factor A (TFAM) (red). In ara-C exposed DRG neurons, TFAM largely co-localizes with the punctate green 8-oxoG immunofluorescence (merged image).