Figure 4. Depletion of mitochondrial content, reduction of mtDNA copy number and reduced expression of mtDNA encoded genes in ara-C exposed DRG neurons.
(A) Following ara-C exposure IF of mitochondrial cytochrome oxidase 1 (COX1) showed reduced signal and aggregated distribution in soma of DRG neurons (green, arrowhead); DRGs are identified by NF200 (red), scale bar = 10 µm. (B) Reduced mtDNA copy number in DRG neurons following ara-C exposure. (C) Expression of mitochondria encoded genes was reduced in the course of ara-C exposure. Bars show changes relative to respective controls. Values represent mean±SEM for four biological experiments; *P<0.05 versus control.