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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Virology. 2018 Mar 31;519:1–11. doi: 10.1016/j.virol.2018.03.021

Figure 10. Met knockdown affects HPV16 gene expression and viral copy number.

Figure 10

Keratinocytes containing the complete episomal HPV16 genome were infected with lentivirus expressing shRNA against Met (Met KD) or a non-target control (NTC), selected, and expanded in culture. a. Representative western blot (n=5) showing knockdown of Met in two independent backgrounds. b. qPCR showing the copy number of viral genomes. c. Southern Blot showing the banding pattern characteristic of episomal genome maintenance, with supercoiled (I) and nicked circular forms (II). d. Cells were cultured in monolayer (Mono) or 1.6% methylcellulose (MC) for 24 hours. Transcript levels for early (E6-7) and late (E1^E4) transcripts were measured using RT-qPCR. *** p<0.001.