Figure 3.

Binding of Pct1-GFP to the Nuclear Membrane Is Associated with PC Levels in Yeast

(A) pct1Δ cells expressing C-terminal GFP-tagged Pct1 were grown to exponential phase (Exp), post-diauxic shift (PDS) phase, or to exponential phase supplemented with 1 mM choline (Exp + Cho), and imaged as described in the STAR Methods. Differential interference contrast (DIC) images are shown for context. Scale bars, 5 μm.

(B) Lipidomic analysis of PC and PE levels at the indicated growth phase. The PE and PC levels shown in nmol (per 50 mg yeast) are relative to internal standards.

(C) PC/PE ratio of WT cells at the indicated growth phase.

Data are means ± SD from three independent experiments. One-way ANOVA with Bonferroni multiple comparison ^∗p < 0.05. See Figures S3 and S4.