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. 2018 Jul 1;439(1):3–18. doi: 10.1016/j.ydbio.2018.04.005

Fig. 2.

Fig. 2

An ectopic notochord graft leads to the formation of ectopic sclerotome lateral to the endogenous somites. A. Schematic showing the notochord graft procedure. (Left = quail donor, centre left = sagittal view of steps 1–4 of notochord graft procedure; centre right = chick host with quail notochord grafted lateral to the host somites (step 5); right = transverse section of chick host after grafting). B–D. WMISH for Pax1 (a marker of the sclerotome) in a HH stage 25 embryo, three days after a notochord graft. Ectopic Pax1 expression is seen in the grafted region anterior to the forelimb (B–C; black arrows). No ectopic Pax1 expression is seen on the contralateral side of the embryo (D). E–G. WMISH for Pax1 in a HH stage 25 embryo, three days after a ‘sham’ notochord graft. No ectopic expression is seen in the operated region (E-F) or on the contralateral side of the embryo (G).