Figure 6.
HTTex1 Forms Liquid-like Assemblies by Liquid-Liquid Phase Separation
(A) Liquid-liquid phase separation of 25QP-GFP induced by molecular crowding. Scale bar, 5 μm.
(B) Fusion of 25QP-GFP droplets. Scale bar, 2 μm. See also Video S5.
(C) Half-bleach (Brangwynne et al., 2009) of a 25QP-GFP droplet (white dashed outline). Kymograph shows redistribution of 25QP-GFP after the bleach. Scale bar, 1 μm.
(D and E) Intermolecular interactions of 25QP-GFP in droplets. (D) Droplet formation at different salt and protein concentrations. The phase diagram indicates conditions where 25QP-GFP forms droplets (blue dots) and where it does not (gray dots). (E) Phase diagram showing the effect of 1,6-hexanediol concentrations on droplet formation. All experiments were carried out in the presence of 10% dextran as a crowding agent, and each condition was assessed at least twice.