Table 3.
Summary statistics for protein and peptide identifications in our study.
| Arabidopsis thaliana | Oryza sativa Indica | |
|---|---|---|
| Identified proteins | 6332 | 2878 |
| Identified peptides | 52,430 | 10,117 |
| Identified phosphoproteins | 3189 | 1613 |
| Identified phosphopeptides | 6537 | 2307 |
| Total phospho-sitesa | 9249 | 2580 |
| Singly phosphorylated proteins | 1342 (42%) | 1025 (63.5%) |
| Multi-phosphorylated proteins | 1847 (58%) | 588 (36.5%) |
| Shared motif containing proteins | 791 (24.80%) | 1012 (62.74%) |
| pS%:pT%:pY%b | 88.3: 11.4: 0.4 | 86.7: 12.8: 0.5 |
| 1Pi%:2Pi%:3Pi%c | 78.9: 18.7: 2.3 | 89.7: 9.2: 1.1 |
| (1Pi%:2Pi%:3Pi%) in serine containing phosphopeptidesd | 78.7: 18.9: 2.4 | 90.9: 8.0: 1.1 |
| (1Pi%:2Pi%:3Pi%) in threonine containing phosphopeptidese | 71.7: 23.8: 4.5 | 87.1: 12.9: 0 |
| (1Pi%:2Pi%:3Pi%) in tyrosine containing phosphopeptidesf | 58.3: 41.7: 0 | 91.7: 8.3: 0 |
Phosphorylation sites assignment with 99% certainty based on a p-value of 0.01 (Ascore ≥ 20).
Relative abundance of serine, threonine, and tyrosine phosphorylation sites based on analyzing 9249 phosphorylation sites in Arabidopsis and 2580 phosphorylation sites in rice.
Relative frequency of singly, doubly, and triply phosphorylated peptides based on analyzing a total of 6537 p-peptides Arabidopsis and 2307 p-peptides in rice - no phosphopeptides with four or more phosphates were found in this study.
Relative frequency of singly, doubly, and triply phosphorylated peptides in serine containing phosphopeptides based on analyzing a total of 7489 serine containing phosphopeptides in Arabidopsis and 2148 serine containing phosphopeptides in rice.
Relative frequency of singly, doubly, and triply phosphorylated peptides in threonine-containing phosphopeptides based on analyzing a total of 1022 threonine containing phosphopeptides in Arabidopsis and 325 threonine-containing phosphopeptides in rice.
Relative frequency of singly, doubly, and triply phosphorylated peptides in tyrosine containing phosphopeptides based on analyzing a total of 36 tyrosine containing phosphopeptides in Arabidopsis and 12 tyrosine containing phosphopeptides in rice.