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. 2018 Jul 15;439(2):69–79. doi: 10.1016/j.ydbio.2018.04.022

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 1 — Optimised LiCl and UV treatments ofX. tropicalisembryos perturb their DV axis determination in line with the original experiments inX. laevis. (A-C) DV perturbations in X. tropicalis embryos caused by LiCl or UV treatments. (A) Phenotypical morphologies. (B) WMISH of dorsal (noggin) and ventral (bmp4) markers in mid-gastrula embryos. (C) RT-qPCR of dorsal (gsc and noggin) and ventral (bmp4 and wnt8a) markers in mid-gastrula embryos. Error bars, standard error of the mean (SEM) of biological triplicates. Student's two-tailed t-test: *, p < 0.1; **, p < 0.05; and ***, p < 0.01. DAI, dorso-anterior index.