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Expression analysis of uncharacterised genes identified by the means of nongenetic axis perturbation and comparative transcriptomics. RT-qPCR (A) and WMISH (B) of c8orf4, Xetrov72022004, K00726 and cpe. (A) Error bars, SEM of biological triplicates. Student's two-tailed t-test: *, p < 0.1; **, p < 0.05; and ***, p < 0.01. (B) Mid-gastrula stages, vegetal and lateral view except for cpe with vegetal view only; and mid-tailbud stage, lateral view.
