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. 2018 Jun;188(6):1389–1405. doi: 10.1016/j.ajpath.2018.02.006

Figure 1.

Figure 1

Injection of JQ1 16 hours after partial hepatectomy (PHx) impaired liver regeneration. A: Schematic for experimental plan for injection of JQ1 (50 mg/kg) or vehicle solution 16 hours after PHx, followed by harvest at 40 to 45 hours after PHx. B: Serum aspartate aminotransferase (AST) levels were significantly elevated in JQ1-injected mice compared with vehicle-injected controls 40 hours after PHx. C: Immunohistochemistry for proliferating cell nuclear antigen (PCNA) and Cyclin D1 revealed significantly reduced hepatocyte proliferation and Cyclin D1 expression in JQ1-injected mice. D: Significantly reduced Ccnd1 gene expression was found in JQ1-injected mice. E: No reduction in total β-catenin levels but a significant reduction in Cyclin D1 protein levels was evident in JQ1-injected mice. F: Significant reduction in Rgn gene expression was found in JQ1-injected mice. G: Significant reduction in Axin2 gene expression was found in JQ1-injected mice. H: Significant reduction in Lect2 gene expression was found in JQ1-injected mice. Data are expressed as means ± SD. ∗P < 0.05, ∗∗P < 0.01 (t-test). Scale bars = 50 μm. CV, central vein; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PV, portal vein.