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. 2018 Jun;188(6):1389–1405. doi: 10.1016/j.ajpath.2018.02.006

Figure 7.

Figure 7

Bromodomain and extraterminal (BET) inhibition impaired hepatocyte-driven liver regeneration in zebrafish. A: Scheme illustrating the periods of acetaminophen (APAP) treatment (I, injury) and liver regeneration (R). Arrows indicate analysis stage. B: Epifluorescence images showing fabp10a:CFP expression in the untreated larvae or larvae treated with APAP. C: Scheme illustrating the periods of APAP or metronidazole (Mtz) treatment and liver regeneration. Arrow indicates analysis stage. D: Confocal images showing the expression of Alcam (green), Tp1:H2B-mCherry (red), and fabp10a:CFP-NTR (gray) in regenerating livers at 24 hours of recovery (R24h). Arrows point to biliary epithelial cell–derived hepatocytes, which weakly expressed Tp1:H2B-mCherry. E: Scheme illustrating the periods of APAP and BET inhibitor treatments. Arrows indicate analysis stages. F: Epifluorescence images showing fabp10a:CFP expression in the regenerating larvae treated with two different BET inhibitors, iBET151 and JQ1. Arrows point to the liver. G: Quantification of fabp10a:CFP expression in the regenerating larvae treated with dimethyl sulfoxide (DMSO) or BET inhibitors, as shown in D. Data are expressed as means ± SEM. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 (one-way analysis of variance). Scale bars = 100 μm (B, D, and F). CFP, cyan fluorescent protein; CTCF, corrected total cell fluorescence; dpf, days after fertilization.