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. 2018 Jun;188(6):1389–1405. doi: 10.1016/j.ajpath.2018.02.006

Figure 8.

Figure 8

Bromodomain and extraterminal (BET) inhibition reduced hepatocyte proliferation in the zebrafish acetaminophen (APAP)-induced injury model. A: Scheme illustrating the periods of APAP and JQ1 treatments and analysis stages (arrows). B: Confocal images showing the hepatic expression of fabp10a:CFP (gray, hepatocytes), Tp1:H2B-mCherry (red, biliary epithelial cells), and fabp10a:mAGFP-gmnn (green, proliferating hepatocytes). C: Quantification of the number of mAGFP-gmnn+ cells, as shown in B. D–H: Real-time quantitative PCR data showing the relative expression levels of ccnd1, pcna, myca, brd4, and axin2 among uninjured control livers at 5 days after fertilization (dpf) and dimethyl sulfoxide (DMSO)- and JQ1-treated regenerating livers at 12 hours of recovery (R12h). Data are expressed as means ± SEM. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗∗P < 0.0001 (one-way analysis of variance). Scale bar = 100 μm. CFP, cyan fluorescent protein.