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. 2018 Jun;188(6):1334–1344. doi: 10.1016/j.ajpath.2018.02.009

Figure 1.

Figure 1

Persistent Myc expression drives tumorigenesis in choroid plexus (ChP). A and B: Immunohistochemical (A) and immunoblot analyses (B) demonstrate higher Myc expression in embryonic day (E) 8.5 forebrain compared to E10.5 forebrain. Dashed lines indicate division between surface ectoderm, mesenchyme (ME), and neuroepithelium (NE) at E8.5, and division between the developing epidermis/meninges and the neuroepithelium at E10.5. C: Lateral ventricle (LV) and fourth ventricle (4V) choroid plexuses from 8- to 9-week–old wild-type (WT) and Myc-overexpressed (OE) mice. White arrows denote tumorigenic region. Note posterior regionalization of tumor in the LV choroid plexus, whereas tumor develops throughout the 4V choroid plexus. D: Hematoxylin and eosin staining shows choroid plexus carcinoma in the LV and 4V choroid plexuses of Myc-OE mice. Arrows indicate mitoses; the boxed areas in left panels are shown at higher magnification in right panels. E: Ki-67 staining shows high proliferation in choroid plexus tumors in both LV and 4V of Myc-OE mice. F: Tumors in both LV and 4V are variably immunoreactive to renal inward rectifier K+ channel (Kir7.1). G and H: The percentage of multiciliated epithelial cells is decreased in both the LV and 4V choroid plexuses in the tumorigenic regions of 8-week–old Myc-OE mice. Solid arrows indicate monociliated cells; open arrows indicate multiciliated cells. Data expressed as means ± SEM (H). ∗P < 0.05 (Welch corrected t-test). Scale bars: 1 mm (C); 500 μm (D, left); 20 μm (D, right; E and F); 10 μm (G). A, anterior; Actb, β-actin; Arl13b, ADP-ribosylation factor-like protein 13b; L, lateral; M, medial; P, posterior.