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. 2018 Jun;188(6):1334–1344. doi: 10.1016/j.ajpath.2018.02.009

Figure 3.

Figure 3

Myc-driven tumorigenesis up-regulates protein biosynthetic machinery. A and B: Nucleolar volume was higher in Myc-overexpressed (OE) choroid plexuses (ChP) than in those of wild-type (WT) mice at 8 weeks in both the lateral ventricle (LV) and fourth ventricle (4V). Outliers excluded by the ROUT method (Q = 1%). Arrows indicate fibrillarin-positive nucleoli. C: Quantitative RT-PCR measures changes in expression of ribosomal subunits (Rps17, Rps5, Rps12, Rpl11, and Rpl10a) in the choroid plexuses of 7- to 9-week–old Myc-OE mice. Note: LV choroid plexus samples contained entire tissue, including nontumorigenic anterior domain. D:O-propargyl-puromycin (OPP) incorporation into WT and Myc-OE tissue. The boxed areas correspond to the insets shown at higher magnification. E: Overall distribution of cells with high protein-synthesis levels, measured by OPP incorporation, was redistributed to higher OPP levels in Myc-OE choroid plexuses than in those of WT mice at 8 weeks in both the LV and 4V choroid plexuses. Data are expressed as means ± SEM (B, C, and E). n = 4 embryos of each genotype from two litters (C). ∗∗∗∗P < 0.0001 (U-test); †P < 0.05, ††P < 0.01 (Kruskal-Wallis test). Scale bars: 10 μm (A); 100 μm (D). RPL, ribosomal protein L; RPS, ribosomal protein S.