Figure 1.

Expression of Notch receptors, cognate ligands, and Tnfsf11 in the spleen and bone marrow (BM). Spleens and femurs were dissected from 7- and 10-week–old wild-type male mice, respectively. Cellular fractions enriched or depleted of CD19-expressing splenocytes (CD19⁺ or CD19⁻, respectively) were obtained from spleen suspensions, and bone marrow cells were harvested from femurs, by centrifugation. Total RNA was extracted from isolated cells, and mRNA was quantified by real-time quantitative RT-PCR in the presence of specific primers. Data are expressed as Notch1, Notch2, Notch3, Notch4, Jag1, Dll3, and Tnfsf11 copy number, corrected for Rpl38 expression. Jag2, Dll1, Dll4, and Tnfrsf11b were not detected in any cell preparation shown. Data are expressed as means ± SD. n = 3 splenocytes; n = 4 bone marrow biological replicates.