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. 2018 Jun;188(6):1430–1446. doi: 10.1016/j.ajpath.2018.02.010

Figure 4.

Figure 4

General appearance of CD19Cre/WT;Notch2ΔPEST/ΔPEST mice and Notch signaling in the spleen and bone marrow. Weight, spleen weight, documentation of Notch2[ΔPEST]COIN inversion, and expression of Notch2 target genes in control and CD19Cre/WT;Notch2ΔPEST/ΔPEST mice. A and B: Body weight (A) and ratio of spleen weight/body weight (B) of 2-month–old control and CD19Cre/WT;Notch2ΔPEST/ΔPEST male and female mice. C: DNA was extracted from spleen or bone marrow cells of 2-month–old male CD19Cre/WT;Notch2ΔPEST/ΔPEST and Notch2[ΔPEST]COIN/[ΔPEST]COIN control mice. Notch2[ΔPEST]COIN inversion was demonstrated by gel electrophoresis of PCR products obtained with primers specific for the Notch2ΔPEST allele. The arrow indicates the position of the 250-bp amplicon. D: Total RNA was extracted from spleen or bone marrow cells of 2-month–old CD19Cre/WT;Notch2ΔPEST/ΔPEST and control Notch2[ΔPEST]COIN/[ΔPEST]COIN mice, and gene expression was measured by real-time quantitative RT-PCR. Notch2ΔPEST, Notch2PEST, Hes1, and Hes5 mRNA copy number corrected for Rpl38 expression are shown. Data are expressed as means ± SD (A, B, and D). n = 3 Notch2[ΔPEST]COIN/[ΔPEST]COIN controls and n = 4 CD19Cre/WT;Notch2ΔPEST/ΔPEST for male mice; n = 4 Notch2[ΔPEST]COIN/[ΔPEST]COIN controls and n = 3 CD19Cre/WT;Notch2ΔPEST/ΔPEST for female mice (A and B); n = 4 Notch2[ΔPEST]COIN/[ΔPEST]COIN controls and n = 3 CD19Cre/WT;Notch2ΔPEST/ΔPEST mice biological replicates (D). ∗P < 0.05 versus control (t-test).