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. 2018 Jun;188(6):1430–1446. doi: 10.1016/j.ajpath.2018.02.010

Figure 5.

Figure 5

B-cell allocation in the spleen of CD19Cre/WT;Notch2ΔPEST/ΔPEST mice. Flow cytometry of spleen cells from control and CD19Cre/WT;Notch2ΔPEST/ΔPEST mice. A: Representative dot plot of flow cytometry of spleen cells from 2-month–old CD19Cre/WT;Notch2ΔPEST/ΔPEST (Notch2ΔPEST) and Notch2[ΔPEST]COIN/[ΔPEST]COIN control male and female sex-matched littermates. Cells were stained with anti-CD21/35 and anti-CD23 antibodies. Frequency of CD21/35highCD23− marginal zone (MZ) B cells and CD21/35intCD23+ follicular B cells gated on B220+/IgM+ B-cell populations is shown. Values represent the percentage of MZ B cells and follicular B cells. B: Histogram of the mean fluorescence intensity (MFI) of the CD21/35 cell population from 2-month–old CD19Cre/WT;Notch2ΔPEST/ΔPEST and Notch2[ΔPEST]COIN/[ΔPEST]COIN control mice. C: Bar graph of MFI of CD21/35 cells for control Notch2[ΔPEST]COIN/[ΔPEST]COIN and CD19Cre/WT;Notch2ΔPEST/ΔPEST mice. D: Representative image of spleen follicles [white pulp (WP)] stained with anti-IgM surrounded by a CD169+ metallophilic macrophage ring, stained with anti-CD169 antibodies, separating the red pulp (RP) and defining the MZ. The presence of nucleated cells was verified by DAPI staining (not shown). Data are expressed as means ± SD (A and C). n = 3 to 4 biological replicates (A); n = 4 biological replicates (C). ∗P < 0.05 versus control.