Figure 6.

General appearance of CD19^Cre/WT;Rosa^Notch1/WT mice and Notch signaling in the spleen and bone marrow. Weight, spleen weight, documentation of Notch1 activation, and expression of Notch1 target genes in control and CD19^Cre/WT;Rosa^Notch1/WT mice. A: Body weight. B: Ratio of spleen/body weight. C: DNA was extracted from spleens of 2-month–old CD19^Cre/WT;Rosa^Notch1/WT and control Rosa^Notch1/WT male and female mice, and excision of the STOP was demonstrated by gel electrophoresis of PCR products obtained with primers specific for the Rosa^Notch1 allele. The arrowheads and arrows indicate the positions of the 500- and 300-bp amplicons, respectively, obtained from the intact or recombined Rosa^Notch1 alleles. D: Total RNA was extracted from spleen or bone marrow cells of 2-month–old CD19^Cre/WT;Rosa^Notch1/WT and control Rosa^Notch1/WT littermates, and gene expression was measured by real-time quantitative RT-PCR. Notch1NICD, Hes1, and Hes5 mRNA copy numbers corrected for Rpl38 expression are shown. Two technical replicates were used for each real-time quantitative RT-PCR. Data are expressed as means ± SD (A, B, and D). n = 7 control and n = 5 CD19^Cre/WT;Rosa^Notch1/WT for male mice; n = 6 control and n = 3 CD19^Cre/WT;Rosa^Notch1/WT for female mice (A and B); n = 8 control and n = 5 CD19^Cre/WT;Rosa^Notch1/WT biological replicates for spleen; n = 8 control and n = 7 CD19^Cre/WT;Rosa^Notch1/WT biological replicates for bone marrow samples (D). ^∗P < 0.05 versus control (t-test).