Skip to main content
. 2018 Jun;188(6):1430–1446. doi: 10.1016/j.ajpath.2018.02.010

Figure 7.

Figure 7

B-cell allocation in the spleen of CD19Cre/WT;RosaNotch1/WT mice. Flow cytometry of spleen cells from control and CD19Cre/WT;RosaNotch1/WT mice. A: Representative dot plot of flow cytometry of spleen cells from 2-month–old CD19Cre/WT;RosaNotch1/WT and control male and female sex-matched littermates. Cells were stained with anti-CD21/35 and anti-CD23 antibodies. Frequency of CD21/35highCD23− marginal zone (MZ) B cells and CD21/35intCD23+ follicular B cells gated on B220+/IgM+ B-cell populations is shown. Values represent the percentage of MZ B cells and follicular B cells. B: Histogram of the mean fluorescence intensity (MFI) of the CD21/35 cell population from 2-month–old CD19Cre/WT;RosaNotch1/WT and RosaNotch1/WT control mice. C: Bar graph of MFI of the CD21/35 cells for control (white bars) and CD19Cre/WT;RosaNotch1/WT (black bars) mice. Data are expressed as means ± SD (A and C). n = 3 to 4 biological replicates (A and C). ∗P < 0.05 versus control (t-test).