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. 2018 Jun;188(6):1430–1446. doi: 10.1016/j.ajpath.2018.02.010

Table 1.

Primers Used for Genotyping and Determination of Cre-Mediated Recombination by PCR

Allele Strand Sequence Amplicon size, bp
Genotyping
 CD19Cre Forward 5′-GCGGTCTGGCAGTAAAAACTATC-3′ 100
Reverse 5′-GTGAAACAGCATTGCTGTCACTT-3′
 CD19WT Forward 5′-CCTCTCCCTGTCTCCTTCCT-3′ 500
Reverse 5′-TGGTCTGAGACATTGACAATCA-3′
 Fabp1 Forward 5′-TGGACAGGACTGGACCTCTGCTTTCC-3′ 200
Reverse 5′-TAGAGCTTTGCCACATCACAGGTCAT-3′
 HprtWT Forward 5′-TTTCTATAGGACTGAAAGACTTGCTC-3′ 200
Reverse 5′-CACAGTAGCTCTTCAGTCTGATAAAA-3′
 HprtCre Forward 5′-GCGGTCTGGCAGTAAAAACTATC-3′ 100
Reverse 5′-GTGAAACAGCATTGCTGTCACTT-3′
 Notch2[ΔPEST]COIN Forward 5′-CCGGGCCGCGACTGAAACCCTAG-3′ 330
Reverse 5′-CCACCACCTCCAGGAGTTGGGC-3′
 Notch2tm1.1ECan Forward Nch2Lox gtF 5′-CCCTTCTCTCTGTGCGGTAG-3′ WT = 310
Notch2tm1.1ECan = 400
Reverse Nch2Lox gtR 5′-CTCAGAGCCAAAGCCTCACTG-3′
 Notch2WT Forward 5′-GCTCAGACCATTGTGCCAACCTAT-3′ 100
Reverse 5′-CAGCAGCATTTGAGGAGGCGTAA-3′
 RosaNotch1 Forward 5′-GGAGCGGGAGAAATGGATATG-3′ WT = 600
RosaNotch1 NICD = 250
Reverse WT 5′-AAAGTCGCTCTGAGTTGTTATTG-3′
Reverse RosaNotch1 NICD 5′-GCGAAGAGTTTGTCCTCAACC-3′
LoxP recombination
 Notch2[ΔPEST]COIN Forward 5′-GTACTTCAGCACAGTTTTAGAGAAC-3′ Not recombined and not detected
Recombined = 250
Reverse 5′-GTGAGTCACCCGCCGGATGTC-3′
 RosaNotch1 Forward 5′-TTCGCGGTCTTTCCAGTGG-3′ Not recombined = 500
Recombined = 300
Reverse absent loxP recombination 5′-AGCCTCTGAGCCCAGAAAGC-3′
Reverse present loxP recombination 5′-GCCGACTGAGTCCTCGCC-3′

NICD, Notch intracellular domain; WT, wild type.