Table 2.

Primers Used for RT-qPCR Determinations

Gene	Sequence	GenBank accession no.
Cre	F: 5′-TGTTAATCCATATTGGCAGAACGA-3′	X03453
	R: 5′-ATCCATCGCTCGACCAGTTTA-3′
Dll1	F: 5′-CTCTTCCCCTTGTTCTAAC-3′	NM_007865
	R: 5′-ACAGTCATCCACATTGTC-3′
Dll3	F: 5′-TCTATCTTGTCCCTTCTCTATCA-3′	NM_007866
	R: 5′-AATCATTCAGGCTCCATCTC-3′
Dll4	F: 5′-TGACAAGAGCTTAGGAGAG-3′	NM_019454
	R: 5′-GCTTCTCACTGTGTAACC-3′
Hes1	F: 5′-ACCAAAGACGGCCTCTGAGCACAGAAAGT-3′	NM_008235
	R: 5′-ATTCTTGCCCTTCGCCTCTT-3′
Hes5	F: 5′-GGAGATGCTCAGTCCCAAGGAG-3′	NM_010419
	R: 5′-TGCTCTATGCTGCTGTTGATGC-3′
Jag1	F: 5′-TGGGAACTGTTGTGGTGGAGTCCG-3′	NM_013822
	R: 5′-GTGACGCGGGACTGATACTCCT-3′
Jag2	F: 5′-AAGGTGGAAACAGTTGT-3′	NM_010588
	R: 5′-CACGGGCACCAACAG-3′
Notch1	F: 5′-GTCCCACCCATGACCACTACCCAGTTC-3′	NM_008714
	R: 5′-GGGTGTTGTCCACAGGTGA-3′
Notch1NICD	F: 5′-GTGCTCTGATGGACGACAAT-3′	NM_008714
	R: 5′-GCTCCTCAAACCGGAACTTC-3′
Notch2PEST	F: 5′-CCATTGTGCCAACCTATCAT-3′	NM_010928
	R: 5′-TTGAGGAGGCGTAACTGT-3′
Notch2ΔPEST	F: 5′-GGCTTTCCCACCTACCAT-3′	Not applicable
	R: 5′-TAGTCGGGCACGTCGTAG-3′
Notch2	F: 5′-TGACGTTGATGAGTGTATCTCCAAGCC-3′	NM_010928
	R: 5′-GTAGCTGCCCTGAGTGTTGTGG-3′
Notch3	F: 5′-CCGATTCTCCTGTCGTTGTCTCC-3′	NM_008716
	R: 5′-TGAACACAGGGCCTGCTGAC-3′
Notch4	F: 5′-CCAGCAGACAGACTACGGTGGAC-3′	NM_010929
	R: 5′-GCAGCCAGCATCAAAGGTGT-3′
Rpl38	F: 5′-AGAACAAGGATAATGTGAAGTTCAAGGTTC-3′	NM_001048057; NM_001048058; NM_023372
	R: 5′-CTGCTTCAGCTTCTCTGCCTTT-3′
Tnfrsf11b	F: 5′-CAGAAAGGAAATGCAACACATGACAAC-3′	NM_009399
	R: 5′-GCCTCTTCACACAGGGTGACATC-3′
Tnfsf11	F: 5′-TATAGAATCCTGAGACTCCATGAAAAC-3′	NM_011613
	R: 5′-CCCTGAAAGGCTTGTTTCATCC-3′

GenBank accession numbers identify the transcripts recognized by primer pairs (https://www.ncbi.nlm.nih.gov/nuccore).

F, forward; R, reverse; RT-qPCR, real-time quantitative RT-PCR.