Table 3.
B-Cell Populations in the Bone Marrow of 2-Month–Old Notch2ΔPEST and RosaNotch1 Mice and Sex-Matched Littermate Controls
| Gate used | B220+ IgM− |
B220+CD19+CD117− |
||
|---|---|---|---|---|
| CD19− CD117− pre–pro-B |
CD19+ CD117+ pro-B |
CD19+ CD117− pre-B |
IgM+ immature B |
|
| Notch2ΔPEST/ΔPEST | ||||
| Male | ||||
| Control | 30.5 ± 5.1 | 0.5 ± 0.1 | 66.0 ± 6.1 | 74.6 ± 3.2 |
| Notch2ΔPEST | 27.2 ± 3.0 | 0.4 ± 0.1 | 69.7 ± 2.9 | 68.8 ± 4.1 |
| Female | ||||
| Control | 29.2 ± 3.7 | 0.5 ± 0.2 | 67.3 ± 3.5 | 71.5 ± 1.6 |
| Notch2ΔPEST | 24.7 ± 4.4 | 0.4 ± 0.0 | 72.9 ± 4.6 | 69.6 ± 5.5 |
| RosaNotch1 | ||||
| Male | ||||
| Control | 20.5 ± 0.9 | 1.1 ± 0.1 | 76.7 ± 1.0 | 66.1 ± 1.8 |
| RosaNotch1 | 26.0 ± 1.7∗ | 0.9 ± 0.1∗ | 72.9 ± 3.4 | 67.3 ± 1.0 |
| Female | ||||
| Control | 26.1 ± 2.3 | 0.8 ± 0.1 | 69.7 ± 4.5 | 67.3 ± 1.2 |
| RosaNotch1 | 31.4 ± 6.0 | 0.8 ± 0.3 | 67.5 ± 7.8 | 73.2 ± 4.0 |
Data are expressed as percentages within the indicated gates and represent means ± SD for three to four biological replicates.
Notch2ΔPEST, CD19Cre/WT;Notch2ΔPEST/ΔPEST; RosaNotch1, CD19Cre/WT;RosaNotch1/WT.
∗
P < 0.05 RosaNotch1 versus control mice by unpaired t-test.