Table 5.
Femoral Microarchitecture Assessed by μCT of 2-Month–Old RosaNotch1 Mice and Sex-Matched Littermate Controls
| Variables | Males |
Females |
||
|---|---|---|---|---|
| Control (n = 7) | RosaNotch1 (n = 5) | Control (n = 6) | RosaNotch1 (n = 3) | |
| Distal femur trabecular bone | ||||
| Bone/total volume, % | 9.3 ± 4.4 | 7.4 ± 3.0 | 5.1 ± 1.5 | 5.0 ± 0.6 |
| Trabecular separation, μm | 188 ± 28 | 199 ± 21 | 228 ± 17 | 227 ± 12 |
| Trabecular no., 1/mm | 5.4 ± 0.8 | 5.1 ± 0.6 | 4.4 ± 0.3 | 4.4 ± 0.2 |
| Trabecular thickness, μm | 33 ± 5 | 32 ± 4 | 30 ± 2 | 28 ± 1 |
| Connectivity density, 1/mm3 | 274 ± 180 | 197 ± 138 | 109 ± 45 | 109 ± 33 |
| Structure-model index | 2.4 ± 0.4 | 2.7 ± 0.3 | 2.8 ± 0.2 | 2.7 ± 0.1 |
| Density of material, mg HA/cm3 | 1023 ± 16 | 1017 ± 16 | 1009 ± 17 | 1014 ± 13 |
| Femoral midshaft cortical bone | ||||
| Bone/total volume, % | 86.6 ± 2.7 | 85.7 ± 2.6 | 86.6 ± 2.9 | 85.6 ± 1.2 |
| Porosity, % | 13.4 ± 2.7 | 14.3 ± 2.6 | 13.4 ± 2.9 | 14.4 ± 1.2 |
| Cortical thickness, μm | 148 ± 20 | 138 ± 19 | 133 ± 17 | 130 ± 7 |
| Total area, mm2 | 1.9 ± 0.2 | 1.7 ± 0.2 | 1.6 ± 0.1 | 1.6 ± 0.1 |
| Bone area, mm2 | 0.8 ± 0.1 | 0.8 ± 0.1 | 0.7 ± 0.1 | 0.7 ± 0.1 |
| Periosteal perimeter, μm | 4.8 ± 0.3 | 4.7 ± 0.2 | 4.5 ± 0.2 | 4.5 ± 0.1 |
| Endocortical perimeter, mm | 3.6 ± 0.2 | 3.5 ± 0.1 | 3.4 ± 0.1 | 3.4 ± 0.1 |
| Density of material, mg HA/cm3 | 1137 ± 14 | 1132 ± 11 | 1158 ± 22 | 1141 ± 1.2 |
μCT was performed at the femoral distal end for trabecular or midshaft for cortical bone. Data are expressed as means ± SD of biological replicates.
μCT, microcomputed tomography; HA, hydroxyapatite; RosaNotch1, CD19Cre/WT;RosaNotch1/WT.