Table 1.
Antibodies used for immunochemistry and flow cytometry.
| Target | Host | Supplier | Dilution | Secondary antibody |
|---|---|---|---|---|
| GFP | Rabbit polyclonal | Millipore | 1 : 250 | 488 anti-rabbit |
| NeuN | Mouse monoclonal | Millipore | 1 : 250 | 594 anti-mouse |
| GFAP | Rabbit polyclonal | Dako | 1 : 500 | 594 anti-rabbit |
| Iba1 | Rabbit polyclonal | Wako | 1 : 500 | 594 anti-rabbit |
| DcX | Rabbit polyclonal | Abcam | 1 : 300 | 594 anti-rabbit |
| β3-Tubulin | Mouse monoclonal | Sigma | 1 : 250 | 594 anti-mouse |
| MAP2 | Chicken monoclonal | Abcam | 1 : 250 | 594 anti-chicken |
| CD34 | Rabbit polyclonal | Abcam | 1 : 50 | 488 anti-rabbit |
| CD44 | Rabbit polyclonal | Abcam | 1 : 50 | 488 anti-rabbit |
| CD73 | Rabbit polyclonal | Abcam | 1 : 110 | 488 anti-rabbit |
GFP: green fluorescent protein; NeuN: neuronal nuclei; GFAP: glial fibrillary acidic protein; Iba1: ionized calcium-binding adaptor molecule 1; DcX: doublecortin; MAP2: microtubule-associated protein 2.