Table 6.

STODI, mutagenicity test results.

Strain	S9	Untreated control	Vehicle control (DMSO)	STODI					Positive control
				78.1 μg/ml	156.2 μg/ml	312.5 μg/ml	625 μg/ml	1250 μg/ml
S. typhimurium
TA 98	−S9	0.67 ± 1.15	0.33 ± 0.58	2.00 ± 2.00	0.67 ± 1.15	1.00 ± 0.00	0.00 ± 0.00	2.33 ± 0.58	48.00∗ ± 0.00
	+S9	2.67 ± 2.08	0.67 ± 0.58	1.33 ± 0.58	0.33 ± 0.58	1.33 ± 1.53	1.00 ± 1.00	1.00 ± 1.00	41.00∗ ± 1.73
TA 100	−S9	2.00 ± 1.00	2.33 ± 0.58	1.67 ± 0.58	1.67 ± 0.58	2.33 ± 1.53	2.33 ± 2.08	0.33 ± 0.58	46.67∗ ± 0.58
	+S9	4.33 ± 1.53	3.00 ± 0.00	3.67 ± 2.08	4.33 ± 4.04	5.33 ± 2.31	2.67 ± 0.58	6.00 ± 2.00	48.00∗ ± 0.00
TA 1535	−S9	2.00 ± 1.73	0.33 ± 0.58	0.33 ± 0.58	0.33 ± 0.58	2.67 ± 1.53	0.33 ± 0.58	1.67 ± 0.58	48.00∗ ± 0.00
	+S9	1.00 ± 1.00	1.00 ± 1.00	1.33 ± 0.58	0.67 ± 1.15	1.67 ± 1.53	1.33 ± 1.15	1.00 ± 0.00	38.33∗ ± 2.08
TA 1537	−S9	0.00 ± 0.00	0.67 ± 1.15	0.67 ± 1.15	0.33 ± 0.58	1.00 ± 1.00	0.33 ± 0.58	0.33 ± 0.58	40.33∗ ± 1.53
	+S9	0.67 ± 0.58	0.67 ± 0.58	2.33 ± 0.58	0.33 ± 0.58	1.67 ± 1.53	0.67 ± 0.58	1.67 ± 0.58	42.67∗ ± 3.21
E. coli
WP2 uvrA	−S9	0.33 ± 0.58	0.00 ± 0.00	0.67 ± 0.58	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	39.33∗ ± 1.53
	+S9	0.67 ± 1.15	0.33 ± 0.58	0.67 ± 1.15	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	34.33∗ ± 4.04

Revertant colonies n = 3 (mean ± SD). ^∗Statistically significant (P < 0.05); positive controls. With S9: 2-aminoanthracene (i) 5 μg/ml, TA 98, TA 100, TA 1535, and TA 1537; (ii) 50 μg/ml, E. coli WP2 uvrA; without S9: 2-nitrofluorene—2 μg/ml, TA 98; 4-nitroquinoline-N-oxide—0.1 μg/ml, TA 100; N⁴-aminocytidine—100 μg/ml, TA 1535; 9-amino-1,2,3,4-tetra hydro acridine hydrochloride—15 μg/ml, TA 1537; 4-nitroquinoline-N-oxide—1.0 μg/ml, E. coli WP2 uvrA.