Figure 1.

Isolation of Hes1 inhibitors. A, procedure for the selection and validation of compounds from the chemical library. HEK293 cells were transfected with the indicated reporter genes expressed under the control of the Hes1 promoter, together with Hes1 under the control of a constitutive promoter (pCMV-Hes1) to repress transcription. B, effect of validated compounds (2.5 μm) on the Hes1-mediated repression of luciferase gene expression. Data are displayed as means ± S.D. Two-tailed Student's t test was used for statistical analysis (*, p ≤ 0.01, and **, p ≤ 0.001, compared with DMSO control). C, effect of validated compounds (10 μm) on the Hes1-mediated repression of EGFPd2 gene expression. Data are mean ± S.D. from a minimum of nine individual fields representing at least 366 cells per condition.