Figure 3.

JI051 induces cell-cycle arrest in G₂/M. A, HEK293 cells expressing the Fucci fluorescent probes mCherry-hCdt1 (30/120) (red) and AmCyan-hGeminin (green) were treated with 1 μm JI051 for 24 h prior to confocal microscopy imaging in live cells. An increase in geminin/Cdt1 ratio indicates an increase in the cell population in the G₂/M phase of the cell cycle. Nocodazole (1000 ng/ml) was used as a positive control. Data are mean ± S.D. from triplicates (nine fields each) representing a minimum of 762 cells per condition. Scale bar, 50 μm. B, calculation of the mitotic index (% of cells with condensed chromatin) following a 24-h incubation with 1 μm JI051 or DMSO. Nuclei were stained with Hoechst 33342. Arrowheads indicate condensed chromatin. Data represent mean ± S.D. from a minimum of 22 individual fields representing at least 373 cells per condition. Scale bar, 10 μm.