Figure 6.

JI130 inhibits MIA PaCa-2 cell growth. A, cells were treated for the indicated periods of time with 1 μm JI130 or DMSO prior to cell proliferation analysis with the tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt. Data represent mean ± S.D. B, dose-response curve of the effect of JI130 and the chemotherapy drug gemcitabine on cell proliferation following a 72-h incubation. Data are expressed as mean ± S.D. C, chemical structures of JI130 and corresponding EC₅₀ value. D, effect of a 24-h incubation with JI130 (100 nm and 1 μm) or DMSO on nucleus appearance. Nuclei were stained with Hoechst 33342. Arrowheads indicate cells with micronuclei. DIC, differential interference contrast. Scale bar, 25 μm.