Figure 13.

C108 selectively inhibits G3BP1 MMA but not G3BP1 Arg SDMA. HEK293T cells were transfected with either empty vector (lanes 1–3) or with FLAG-tagged G3BP1 + cRIG-I (lanes 4–14), and treated with either DMSO vehicle (lanes 1–6) or C108 (lanes 7–14) as indicated. Following immunoprecipitation with anti-FLAG antibody, aliquots of immunoprecipitates were analyzed by Western blotting for either MMA (A), SDMA (B), or FLAG-tagged proteins (C). Note that C108 in the 3–10 μm range inhibited G3BP1 MMA (A) without altering SDMA accumulation (B) or total protein accumulation (C).