Figure 3.

Effects of H₂O₂ on mitochondrial bioenergetics. A and B, the effects of H₂O₂ (●) at 30 μm (A) and 100 μm (B) on the OCR in isolated pancreatic acinar cells compared with controls without H₂O₂ addition (□). C–G, a respiratory function “stress” test was carried out using sequential addition of oligomycin (Oligo, 1 μg/ml), FCCP (0.3 μm), and rotenone (Rot)/antimycin A (Anti A) combined (1 μm) injected sequentially. Acute effects of H₂O₂ (10–100 μm) on baseline changes of OCR (C) and ECAR (D) after 5 min (black) and 30 min (gray), ATP turnover capacity (E), spare respiratory capacity (F), and proton leak (G). The data are shown as the means ± S.E. (n = 3). Significant differences from the control are shown as follows: *, p < 0.05; **, p < 0.01; and ***, p < 0.001.