Figure 8.

Effects of cyclophilin D knockout (Ppif^−/−) and pharmacological inhibition on H₂O₂-induced mitochondrial depolarization and reduction of NADH. A–D, the effects of 50 μm (A and B) and 500 μm (C and D) H₂O₂ on mitochondrial membrane potential (ΔΨ_m) and NADH autofluorescence in pancreatic acinar cells isolated from Ppif^−/− (gray) and WT (C57Bl6, black) mice. The effects of cyclosporine A (CsA) treatment on changes in WT to H₂O₂ are also shown. Changes are expressed as normalized decreases of TMRM fluorescence and falls of NADH from basal levels (F/F₀); the mitochondrial uncoupler CCCP (10 μm) was applied to show maximal depolarization. E and F, the concentration-dependent rates of fall of TMRM fluorescence and NADH autofluorescence in response to H₂O₂ under the various conditions are displayed. All data are shown as the means ± S.E. (averages of ≥80 cells from >4 animals).