Figure 4.

PHLPP2 and GRK5 interact in NRVMs. A, IP of NRVM extracts (150 μg) treated with or without PE (50 μm) for 30 min with antibodies for IgG or GRK5. PHLPP2 (150 kDa) was blotted (IB) for binding and GRK5 (68 kDa) for IP control. The endogenous expression of PHLPP2 was blotted as control for input (bottom panel). B, quantitation of bound PHLPP2 to GRK5 with and without PE stimulation (∼30% decrease versus Con (−)). The graph represents n = 3 independent experiments with biological replicates. **, p < 0.01 versus nontreated (means ± S.D.).