Figure 8.

Modulators acting at sites other than the benzodiazepine site at WT and cysteine-substituted GABA_A receptors are unaffected by DTT treatment. Before modulator effects were tested, the EC_5–10 concentration of GABA was determined in each oocyte. Effects of 200 mm ethanol and 100 nm allopregnanolone were measured in WT and α₁(K104C)β₂γ₂(D75C) GABA_A receptors before (white symbols and bars) and after (dark symbols and bars) application of DTT with no significant changes being observed (two-way ANOVAs; 200 mm ethanol (F(3,43) = 0.031); 100 nm allopregnanolone (F(3,45) = 0.176). Each symbol represents the percent potentiation of the GABA EC_5–10 seen in one oocyte, and each bar represents the mean percent potentiation. Error bars represent the S.E.