Figure 3.

ADAM9 interacts with sorting nexin 9 and 18. A and B, HEK293-VnR cells were transiently transfected with mouse ADAM9, treated with the dithiobis(succinimidyl propionate) cross-linker to stabilize and trap protein-protein interactions in their correct cellular compartments, and lysed. A, lysate was subjected to IP with anti-ADAM9 or IgG control antibodies, examined by SNX9 and SNX18 Western blotting, and re-blotted with anti-ADAM9 antibody. Blots are representative of n = 3 independent experiments. B, lysate was subjected to IP with IgG control or anti-SNX9 antibody and examined as in A. Blots are representative of n = 3 independent experiments. C, HEK293-VnR cells were transiently transfected with mADAM9 and SNX18-tGFP. Lysate was subjected to IP with IgG control or anti-GFP antibody and examined as in A. Blots are representative of n = 3 independent experiments. IB, immunoblot.