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. 2018 Apr 5;293(21):8077–8088. doi: 10.1074/jbc.RA117.001077

Figure 4.

Figure 4.

Redundant effects of sorting nexin 9 and 18 on ADAM9 endocytosis. A, D, and G, MDA-MB-231 cells were transiently transfected with SNX9, SNX18, SNX9 + -18, or control siRNA. Cells were surface labeled with cleavable biotin at 4 °C, left alone to internalize or recycle, and examined by Western blotting as described in the legends to Figs. 1 and 2. B and C, quantification of internalized and recycled ADAM9 in SNX9 knockdown cells from A. Internalized ADAM9 was normalized to total surface ADAM9, whereas recycled ADAM9 was normalized to internalized ADAM9 for either SNX9 or control siRNA. E and F, quantification of internalized and recycled ADAM9 in SNX18 knockdown cells from D was done as in B and C. H and I, quantification of internalized and recycled ADAM9 in SNX9 + -18 knockdown cells from G was described in B and C. Western blots are representative of n = 4 independent experiments. Plots show individual data, and average values ± S.D., *, p < 0.05 (Student's t test).