Figure 2.

TORC2–Gad8 represses transcription of genes located close to the telomeres of chromosome I and II. A, RNA-Seq analysis shows extensive similarity between the transcriptional profiles of Δgad8 and Δtor1. The genes that are up-regulated or down-regulated by at least log₂-fold are presented in Venn diagrams. B, up-regulated genes in Δgad8 and Δtor1 cells are clustered at the subtelomeric regions of chromosome I and II. The log₂ ratio of the distribution of the Δtor1 versus WT (left) or Δgad8 versus WT (right) is plotted as a function of the location of the chromosome. The chromosome number is indicated above each plot. Genes that had a ratio >2, adjusted p value <0.05, and a count of at least 20 in at least one of the samples are shown in red. The arrows indicate clusters of genes along the arms of chromosomes I or II that are found to be abnormally up-regulated in Dgad8 and Δtor1 cells. These clusters are not associated with heterochromatic structure.