Figure 5.

Efforts to rescue growth of the PfmRPL13 knockdown line in the absence of aTc via three approaches. A, Qd was unable to rescue growth of the knockdown parasites in the absence of aTc. B, provision of episomal copies of yDHODH in the knockdown parasites failed to fully rescue growth without aTc over the long term. C, supplementing the knockdown parasites with episomal copies of PfmRPL13–3Myc successfully rescued growth in the absence of aTc. For all conditions, parasitemia and growth index were defined using the same method as descried in Fig. 2C. Data shown are the mean ± S.D. of n = 3 biological replicates.