Table 1.
Affinity measurement for MelB
KD values were measured by intrinsic protein fluorescence titration (Fluorescence) and by isothermal titration microcalorimetry (ITC).
| Fluorescence |
ITC |
|||||||
|---|---|---|---|---|---|---|---|---|
| KD | R2 | KD | n | Enthalpy (ΔH) | Entropy (ΔS) | Entropic contribution (−TΔS) | Free enthalpy (ΔG) | |
| μm | μm | cal/mol | cal/mol/deg | cal/mol | cal/mol | |||
| Galactinol | 0.010 ± 0.001 | 0.99 | 0.12 ± 0.03 | 0.83 | −6908 | 8 | −2356 | −9264 |
| Melibiose | 0.072 ± 0.004 | 0.99 | 0.76 ± 0.11 | 0.94 | −6412 | 6.1 | −1794 | −8206 |
| Raffinose | 0.347 ± 0.42 | 0.99 | 2.9 ± 0.5 | 0.97 | 2115 | 32.5 | −9527 | −7412 |
| Galactose | 13.8 ± 3 | 0.99 | 24 ± 2 | 1 | −797 | 18.4 | −5393 | −6190 |
| Stachyose | 24.6 ± 2.4 | 0.99 | Not determined | |||||