TABLE 1.
Differential gene expression in B. abortus 2308::ΔybeYa
| Functional category and designation | Description | Fold expression change (ΔybeY mutant vs strain 2308) |
|---|---|---|
| Membrane proteins and transport systems | ||
| BAB1_0114 | Glycosyl transferase | 3.3 |
| BAB1_0372 | TRAP dicarboxylate transporter, DctM subunit | −3.2 |
| BAB1_0373 | TRAP-type mannitol/chloroaromatic compound transport system | −3.9 |
| BAB1_1589 | Major facilitator transporter | 3.2 |
| BAB1_1679 | MotA/TolQ/ExbB proton channel | 3.5 |
| BAB1_1680 | Biopolymer transport protein ExbD/TolR | 3.5 |
| BAB1_1681 | Cell envelope biogenesis protein TonB | 3.8 |
| BAB1_1792 | Leu/Ile/Val-binding family protein | −4.5 |
| BAB2_0242 | Putative sulfite oxidase subunit YedZ | 3.9 |
| BAB2_0277 | Choline dehydrogenase and related flavoproteins | −16.1 |
| BAB2_0278 | ABC transporter, permease | −9.5 |
| BAB2_0279 | Inner membrane translocator | −10.3 |
| BAB2_0280 | Shikimate kinase | −8.2 |
| BAB2_0281 | ABC transporter ATPase | −9.9 |
| BAB2_0282 | Leu/Ile/Val-binding family protein | −7.5 |
| BAB2_0300 | Inner membrane translocator | −3.2 |
| BAB2_0519 | Periplasmic spermidine/putrescine-binding protein | −3.3 |
| BAB2_0547 | Solute-binding family 1 protein | −3.7 |
| BAB2_0548 | Vacuolar H+-transporting two-sector ATPase subunit C | −5.7 |
| BAB2_0583 | Aromatic amino acid permease | −3.2 |
| BAB2_0584 | Binding protein-dependent transport system inner membrane protein | −4.0 |
| BAB2_0585 | Solute-binding family 1 protein | −3.2 |
| BAB2_0593 | Leu/Ile/Val-binding family protein | −3 |
| BAB2_0700 | Solute-binding family 5 protein | −4.1 |
| BAB2_0822 | Leu/Ile/Val-binding family protein | −6.9 |
| BAB2_0827 | ABC transporter ATPase | −4.1 |
| BAB2_0828 | Glutelin | −4.1 |
| BAB2_0829 | Inner membrane translocator | −5.6 |
| BAB2_0830 | Leu/Ile/Val-binding family protein | −5.4 |
| BAB2_1109 | d-Xylose ABC transporter | −4.8 |
| DNA replication, transcription, and translation | ||
| BAB1_0636 | Response regulator receiver/transcriptional regulatory protein, C terminal | 3.1 |
| BAB1_1100 | Phage integrase | 3.0 |
| BAB1_1362 | Periplasmic binding protein/LacI transcriptional regulator | −3.3 |
| BAB1_1588 | MarR family regulatory protein | 4.6 |
| BAB2_0222 | Response regulator receiver/transcriptional regulatory protein, C terminal | 3.3 |
| BAB2_1099 | Response regulator receiver/transcriptional regulatory protein, C terminal | 3.2 |
| Flagellum related | ||
| BAB2_0299 | Flagellar hook-length control protein | −3.1 |
| BAB2_1106 | Flagellin, C terminal; flagellin, N terminal | 3 |
| Metabolism, signaling, and enzymatic processes | ||
| BAB1_0204 | Zinc-containing alcohol dehydrogenase | −4 |
| BAB1_0303 | Urease accessory protein UreG | 3.2 |
| BAB1_0459 | Transglycosylase-associated protein | −3.0 |
| BAB1_0577 | Choline dehydrogenase | −3.5 |
| BAB1_0637 | ATPase-like ATP-binding protein | 3.3 |
| BAB1_0646 | Endonuclease/exonuclease/phosphatase family protein | 3.2 |
| BAB1_0867 | Hlyoxalase/bleomycin resistance protein/dioxygenase | 3.4 |
| BAB1_1070 | NAD[P]H dehydrogenase | −4.0 |
| BAB1_1299 | Sugar fermentation stimulation protein A | 3.1 |
| BAB1_1461 | SLT domain-containing protein | 3.8 |
| BAB1_1578 | Glutathione S-transferase | 3.3 |
| BAB1_1855 | GCN5-related N-acetyltransferase | 3.6 |
| BAB1_2001 | Aquaporin Z | −3.1 |
| BAB1_2052 | Luciferase | 3.5 |
| BAB2_0243 | Putative sulfite oxidase subunit YedY | 3 |
| BAB2_0821 | Zinc-containing alcohol dehydrogenase | −4.7 |
| BAB2_0823 | Aldehyde dehydrogenase | −4.9 |
| BAB2_0824 | Glucose-methanol-choline oxidoreductase; GMC oxidoreductase | −3.6 |
| BAB2_0825 | Shikimate/quinate 5-dehydrogenase | −4.6 |
| BAB2_0826 | 3-Ketoacyl-(acyl carrier protein) reductase | −4.1 |
| BAB2_0831 | Zinc-containing alcohol dehydrogenase superfamily protein | −4.2 |
| BAB2_0890 | Ribonucleotide reductase stimulatory protein | −3.0 |
| BAB2_0905 | Cytochrome c heme-binding site; 4Fe-4S ferredoxin, iron-sulfur binding domain | −3.2 |
| BAB2_0906 | Nitrate reductase, delta subunit | −3.2 |
| BAB2_0907 | Nitrate reductase, gamma subunit | −3.3 |
| BAB2_1073 | Immunoglobulin/major histocompatibility complex | 3.7 |
| Hypothetical | ||
| BAB1_0147 | Hyp | 3.5 |
| BAB1_0265 | Hyp | −7.0 |
| BAB1_0266 | Hyp | −3.6 |
| BAB1_0418 | Hyp | 6.2 |
| BAB1_0419 | Hyp | 3.4 |
| BAB1_0420 | Hyp | 4.6 |
| BAB1_1296 | Hyp | −3.8 |
| BAB1_1302 | Hyp | 4.3 |
| BAB1_1341 | Hyp | 4 |
| BAB1_1347 | Hyp | 3.3 |
| BAB1_1509 | Hyp | 3.3 |
| BAB1_1793 | Hyp | −3.2 |
| BAB1_1893 | Hyp | −6.8 |
| BAB1_2156 (ybeY) | Hyp | −12.1 |
| BAB2_0223 | Hyp | 5.2 |
| BAB2_0224 | Hyp | 4 |
| BAB2_0276 | Hyp | −5.0 |
| BAB2_0732 | Hyp | −3.2 |
| BAB2_0740 | Hyp | 4.3 |
| BAB2_0759 | Hyp | −3.2 |
| BAB2_0847 | Hyp | −3.0 |
a
Microarray analysis was performed using total cellular RNA from Brucella strains grown in rich media to late exponential phase, and those genes whose expression was shown to have been altered more than 3-fold in the ybeY deletion strain compared to strain 2308 are shown in the list. Boldface indicates data related to genes previously observed to be required for efficient Brucella infection or as being differentially expressed during intracellular trafficking of Brucella. Hyp, hypothetical protein.