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. 2018 May 24;200(12):e00135-18. doi: 10.1128/JB.00135-18

FIG 2.

FIG 2

Vfr regulates ExlA secretion and exlBA expression. (A) The secretion of ExlA by the indicated strains grown in LB medium. The VBM strain carries a mutation in the VBS present in the exlBA promoter. Immunoblots of 100-fold-concentrated secretomes revealing the ExlA protein and FliC, used as a loading control. (B) RT-qPCR analysis of exlB and exlA relative expression in the wild-type (WT), the vfr mutant, and complemented strains grown in LB medium. The rpoD gene was used as a reference. The experiments were performed in triplicate, and the error bars indicate the standard errors of the means. The P value was determined using the Mann-Whitney U test and is indicated by * (P < 0.05) or ** (P < 0.01) when the difference between the mutant strain and the wild-type or complemented strains is statistically supported.