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. 2018 May 24;200(12):e00086-18. doi: 10.1128/JB.00086-18

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FIG 5 — Recombinant IreP can promote dephosphorylation of CroS. Membrane fractions containing CroS-HA were used as substrates in reactions with recombinant IreK and IreP and analyzed by Phos-tag SDS-PAGE and immunoblot analyses. Membranes from a wild-type strain were incubated with recombinant IreK (the cytoplasmic kinase domain of IreK) and ATP, while membranes from an ΔireP mutant were incubated with recombinant IreP. Results from a 60-min incubation with recombinant IreK and a 10-min incubation with recombinant IreP are shown. Images are representative of results from ≥2 experiments. Strains were SB35(pSLK134) and JL457(pSLK134).