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GFP-PHPKB is recruited to forming macropinosomal cups and is transiently associated with completed macropinosomes. Cells expressing GFP-PHPKB were allowed to attach to coverslips and then bathed in HL5 containing 2 mg/ml Texas Red dextran. Images were captured of a single cell every 5 s by confocal microscopy as described in MATERIALS AND METHODS. Selected images are shown. Bar, 5 μm.
