Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
GFP-ABD is still recruited to the plasma membrane independent of PI 3-kinase activity. Wild-type cells expressing GFP-ABD were incubated in HL-5 medium containing LY294002 at a final concentration of 15 μM. Images were collected by confocal microscopy 10 min after the addition of the drug and represent 10-s intervals. The arrows point to invaginations that began, but the two ends did not fuse to give rise to a macropinosome. Bar, 10 μm.
