Table 1.
Sequences of the primers used for genotyping in this study that investigated the relationship between polymorphisms in the C-C motif chemokine receptor 5 (CCR5) and uridine diphosphate- glucuronosyltransferase 1A (UGT1A) genes and hepatitis B virus infection in two ethnic minority Chinese groups compared with a Han Chinese group.
Gene and loci | Upstream primer | Downstream primer | Product length, base pairs |
---|---|---|---|
UGTA1 rs8175347 | 5′-CTACATAGTCGTCCTTCTTC-3′ | 5′-ACAGTATCTTCCCAGCAT-3′ | 433 |
UGT1A6 rs2070959 | a:5′-TAAGGAGAGCAAGTTTGATGCTC-3 | 5′-GTCTGGGCTTCTGCTGATTGT-3′ | 145 |
b:5′-GGTTTTCCGTGTTCCCTGGAGGATG-3 | 5′-CTCTTGAGGACAGCTGATGC-3′ | 200 | |
c:5′-TAAGGAGAGCAAGTTTGATGCTC-3′ | 5′-CTCTTGAGGACAGCTGATGC-3′ | 300 | |
CCR5-59029 rs1799987 | 5′-GAAGAATCCTGCCACCTAT-3′ | 5′-CTCTGCTCATCCCACTACA-3′ | 559 |
CCR5△32 rs333 | 5′-AGGTCTTCATTACACCTGCAGC-3′ | 5′-CTTCTCATTTCGACACCGAAGC-3′ | 169 (wild-type)137 (mutant) |