TABLE 1.
Primers used for amplification and sequencing
| Oligonucleotide name | Used for: (patient IDa/isolate) | Sequence (5′→3′) |
|---|---|---|
| CRISPR1 seq-F | All patients and isolates | GAAGACTCTATGATTTACCGC |
| CRISPR1 seq-R | All patients and isolates | CAGCAATCACTAAAAGAACCAAC |
| Sp803F | W1/All isolates | TCTAATGTATACTGTAACTGAGGCAG |
| Sp504F | W4/All isolates | TTTATCCCTAGTAGGTT |
| Sp539F | W8/A2 | TCTAAGTGCTCGACCATC |
| Sp8R | W8/A2 | TATCCATCTCGGTGAGATGAGAATTAGCTT |
| Sp31R | W1/All isolates | ATCAGTACCAACAAATGATTTTGTACCATC |
| Sp36R | W3/All isolates | ACATATCCTTTTGTTAGGTCAAAAGAAGAT |
| Sp177R | W9/All isolates | AGTTACTGTTGAGGGTAGTCC |
| 275F | W3/All isolates | ACAGACAAAGAAGATGGCAAG |
a
Patients are identified by “W” followed by a number, i.e., W1 is woman 1.