FIG 7.

RVFV Np/Gn FMIA enables differentiation of vaccinated from infected sheep. Sheep were initially vaccinated with a Gn/Gc subunit vaccine and then challenged with Ken06 and maintained for 7 days. The subunit vaccine was given at 0 days postvaccination (DPV) and then boosted at 21 DPV. Detection of IgG antibodies in sera against the Gn target (A) and the Np target (B) is shown. At 35 DPV, all sheep were challenged (0 DPC). IgG production in sera was monitored using the Gn target (C) and the Np target (D). Np served as the DIVA-compatible marker.